Journal article
Assembly of the Type II Secretion System such as Found in Vibrio cholerae Depends on the Novel Pilotin AspS
RA Dunstan, E Heinz, LC Wijeyewickrema, RN Pike, AW Purcell, TJ Evans, J Praszkier, RM Robins-Browne, RA Strugnell, KV Korotkov, T Lithgow
Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2013
Abstract
The Type II Secretion System (T2SS) is a molecular machine that drives the secretion of fully-folded protein substrates across the bacterial outer membrane. A key element in the machinery is the secretin: an integral, multimeric outer membrane protein that forms the secretion pore. We show that three distinct forms of T2SSs can be distinguished based on the sequence characteristics of their secretin pores. Detailed comparative analysis of two of these, the Klebsiella-type and Vibrio-type, showed them to be further distinguished by the pilotin that mediates their transport and assembly into the outer membrane. We have determined the crystal structure of the novel pilotin AspS from Vibrio chol..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. W-31-109-Eng-38. This work was supported by the National Health & Medical Research Council (NHMRC) Program Grant (606788, to TL and RAS), an Australian Research Council Super Science Grant (to TL and RAS) and by NIH Grant Number 2P20 RR020171 from the National Centre for Research Resources. RAD is supported by a Monash Research Scholarship, AWP is an NHMRC Senior Research Fellow, TL is an ARC Federation Fellow. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.